کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2430330 1106560 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Histone H2A as a transfection agent in crayfish hematopoietic tissue cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Histone H2A as a transfection agent in crayfish hematopoietic tissue cells
چکیده انگلیسی

We report a novel and highly efficient dsRNA transfection system based on one of the nuclear proteins, namely, histone H2A. RT-PCR semi-quantitative analysis of silencing target gene shows that the transfection efficiency of histone H2A is higher than Effectene or liposome-based transfection systems. Importantly, the high efficiency of histone H2A was associated with very low toxicity to the transfected crayfish hematopoietic tissue (Hpt) cells. The non-toxicity, effectiveness and specificity of histone H2A as a transfection agent provides a cheap, simple, highly efficient and reproducible gene delivery system, particularly for the sensitive cell cultures of crustacean animals such as crayfish and shrimp.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 31, Issue 4, 2007, Pages 340–346
نویسندگان
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