کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2430344 | 1106561 | 2008 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Noble tandem-repeat galectin of Manila clam Ruditapes philippinarum is induced upon infection with the protozoan parasite Perkinsus olseni Noble tandem-repeat galectin of Manila clam Ruditapes philippinarum is induced upon infection with the protozoan parasite Perkinsus olseni](/preview/png/2430344.png)
SummaryThe galectin family of lectins plays crucial roles in the innate immunity systems of vertebrates and invertebrates. Noble galectin (MCGal) was cloned from the marine invertebrate Ruditapes philippinarum and characterized. This protein has an open reading frame of 918 nucleotides, with 309 amino acid residues, and a predicted molecular weight of 33.9 kDa. Similar to other galectins, MCGal has neither a signal peptide nor a transmembrane domain, but it contains tandemly repeated carbohydrate recognition domains (CRDs), with typical conserved motifs that are important for carbohydrate recognition. Carbohydrate recognition by the recombinant MCGal (rMCGal), as determined by hapten inhibition of hemagglutination, revealed that rMCGal has features common to the galectin family, i.e., significant affinity for galactose and N-acetylgalactosamine. MCGal mRNA expression was detected mainly in the heart, mantle, foot, adductor, palp, and siphon tissues. Immunohistochemistry (IHC) using an anti-MCGal antibody confirmed MCGal expression in these tissues and in hemocytes. Temporal expression of MCGal mRNA in Manila clams challenged with Perkinsus or Vibrio species was up-regulated as compared with non-challenged healthy clams. rMCGal agglutinated Vibrio tapetis, and agglutination was inhibited by incubation with α-lactose. rMCGal also bound to the surface of Perkinsus olseni. MCGal plays a crucial role in Manila clam defense, particularly with respect to pathogen recognition.
Journal: Developmental & Comparative Immunology - Volume 32, Issue 10, 2008, Pages 1131–1141