کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2430749 | 1553623 | 2016 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A transmembrane C-type lectin receptor mediates LECT2 effects on head kidney-derived monocytes/macrophages in a teleost, Plecoglossus altivelis A transmembrane C-type lectin receptor mediates LECT2 effects on head kidney-derived monocytes/macrophages in a teleost, Plecoglossus altivelis](/preview/png/2430749.png)
• We produced a refolded recombinant PaCLR for anti-PaCLR IgG preparation.
• PaCLR blockage inhibited the effect of PaLECT2 on ayu MO/MФ cytokine expressions.
• PaCLR blockage inhibited the chemotactic effect of PaLECT2 on ayu MO/MФ.
• PaCLR blockage inhibited the effect of PaLECT2 on ayu MO/MФ biological functions.
• PaCLR mediates the immunomodulatory effects of PaLECT2 on ayu MO/MФ.
Leukocyte cell–derived chemotaxin 2 (LECT2) is a multifunctional cytokine involved in many diseases in which immune dysfunction is present. Ayu LECT2 (PaLECT2), which interacts with a C-type lectin receptor (PaCLR), was shown to activate ayu head kidney-derived monocytes/macrophages (MO/MΦ) to improve the outcomes of fish upon bacterial infections. However, it is not known if PaCLR mediates PaLECT2 effects on ayu MO/MΦ. In this study, we determined the role of PaCLR in signal transduction of PaLECT2 on ayu MO/MΦ. We expressed the PaCLR ectodomain in Escherichia coli and produced a refolded recombinant protein (rPaCLR) that was then used to produce the anti-PaCLR IgG (anti-PaCLR) for neutralization. Addition of the refolded PaLECT2 mature peptide (rPaLECT2m) to ayu MO/MΦ cultures, increased cytokine expression, induced chemotaxis, and enhanced phagocytosis and bactericidal activity of these cells were observed. When we added anti-PaCLR to block the ectodomain of PaCLR, these effects were significantly inhibited. Based on our previous works and the data presented here, we conclude that PaCLR mediates the immunomodulatory effects of PaLECT2 on ayu MO/MΦ, thus defining a mechanism by which LECT2 protects fish against pathogens.
Journal: Fish & Shellfish Immunology - Volume 51, April 2016, Pages 70–76