کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2430999 1106741 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Recombinant Saccharomyces cerevisiae serves as novel carrier for oral DNA vaccines in Carassius auratus
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Recombinant Saccharomyces cerevisiae serves as novel carrier for oral DNA vaccines in Carassius auratus
چکیده انگلیسی


• Recombinant Saccharomyces cerevisiae can be engineered to orally deliver gene to Carassius auratus.
• Yeast-delivered gene can be expressed in the intestinal cells of C. auratus.
• Expression of yeast-delivered gene induces stronger antibody response.

Oral delivery of DNA vaccines represents a promising vaccinating method for fish. Recombinant yeast has been proved to be a safe carrier for delivering antigen proteins and DNAs to some species in vivo. However, whether recombinant yeast can be used to deliver functional DNAs for vaccination to fish is still unknown. In this study, red crucian carp (Carassius auratus) was orally administrated with recombinant Saccharomyces cerevisiae harboring CMV-EGFP expression cassette. On day 5 post the first vaccination, EGFP expression in the hindgut was detected under fluorescence microscope. To further study whether the delivered gene could induce specific immune responses, the model antigen ovalbumin (OVA) was used as immunogen, and oral administrations were conducted with recombinant S. cerevisiae harboring pCMV-OVA mammalian gene expression cassette as gene delivery or pADH1-OVA yeast gene expression cassette as protein delivery. Each administration was performed with three different doses, and the OVA-specific serum antibody was detected in all the experimental groups by western blotting and enzyme-linked immunosorbent assay (ELISA). ELISA assay also revealed that pCMV-OVA group with lower dose (pCMV-OVA-L) and pADH1-OVA group with moderate dose (pADH1-OVA-M) triggered relatively stronger antibody response than the other two doses. Moreover, the antibody level induced by pCMV-OVA-L group was significantly higher than pADH1-OVA-M group at the same serum dilutions. All the results suggested that recombinant yeast can be used as a potential carrier for oral DNA vaccines and would help to develop more practical strategies to control infectious diseases in aquaculture.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 47, Issue 2, December 2015, Pages 758–765
نویسندگان
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