کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2431606 | 1106764 | 2014 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Modulation of proteome expression by F-type lectin during viral hemorrhagic septicemia virus infection in fathead minnow cells Modulation of proteome expression by F-type lectin during viral hemorrhagic septicemia virus infection in fathead minnow cells](/preview/png/2431606.png)
• An F-type lectin, RbFTL-3 increases viability of VHSV infected fish cells.
• Protein expressions were modulated by RbFTL-3 in the VHSV infected cells.
• RbFTL-3 inhibits a vascular-sorting protein associated with viral budding process.
• The expression of RbFTL-3 diminishes the loss of prothrombin.
Lectins found in fish tissues play an important role in the innate immune response against viral infection. A fucose-binding type lectin, RbFTL-3, from rock bream (Oplegnathus fasciatus) was identified using expressed sequence tag (EST) analysis. The expression of RbFTL-3 mRNA was higher in intestine than other tissues of rock bream. To determine the function of RbFTL-3, VHSV-susceptible fathead minnow (FHM) cells were transfected with pcDNA3.1(+) or pcDNA3.1(+)-RbFTL-3 and further infected with VHSV. The results show that the viability of FHM cells transfected with pcDNA3.1(+)-RbFTL-3 is higher than that of cells transfected with pcDNA3.1(+) (relative cell viability: 28.9% vs 56.2%). A comparative proteomic analysis, performed to explore the proteins related to the protective effect of RbFTL-3 in the cells during VHSV infection, identified 90 proteins differentially expressed in VHSV-infected FHM cells transfected with pcDNA3.1(+) or pcDNA3.1(+)-RbFTL-3. The expression of RbFTL-3 inhibits a vascular-sorting protein (SNF8) and diminishes the loss of prothrombin, which are closely associated with controlling viral budding and hemorrhage in fish cells, respectively. Subsequent Ingenuity Pathways Analysis enabled prediction of their biofunctional groupings and interaction networks. The results suggest RbFTL-3 modulates the expression of proteins related to viral budding (SNF8, CCT5 and TUBB) and thrombin signaling (F2) to increase the viability of VHSV infected cells.
Journal: Fish & Shellfish Immunology - Volume 39, Issue 2, August 2014, Pages 464–474