کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2431775 | 1106772 | 2013 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A heat shock protein 90 β isoform involved in immune response to bacteria challenge and heat shock from Miichthys miiuy A heat shock protein 90 β isoform involved in immune response to bacteria challenge and heat shock from Miichthys miiuy](/preview/png/2431775.png)
• A complete HSP90 β isoform has been cloned and identified from Miichthys miiuy.
• HSP90 genes are under purifying selection pressure among fish species.
• Bacteria challenge and heat shock test imply HSP90 involved in stress response of M. miiuy.
• Expression analysis of HSP90 protein is investigated in M. miiuy.
Heat shock protein 90 (HSP90) is highly conserved molecular chaperone that plays a critical role in cellular stress response. In this study, we reported the identification and functional analysis of a heat shock protein 90 gene from miiuy croaker (designated Mimi-HSP90). Mimi-HSP90 contained five conserved HSP90 protein family signatures and shared 89.6%–99.5% similarity with other known HSP90 β isoform. Homology analysis and structure comparison further indicated that Mimi-HSP90 should be β isoform member of the HSP90 family. The molecular evolutionary analysis showed that HSP90 was under an overall strong purifying select pressure among fish species. Mimi-HSP90 gene was constitutively expressed in ten examined tissues, and the expression level of liver was higher than in other tissues. The expression level of Mimi-HSP90 gene under bacterial infection and heat shock were analyzed by real-time quantitative RT-PCR, resulted in significant changes in liver, spleen, and kidney tissues. The purified recombinant pET-HSP90 protein was used to produce the polyclonal antibody in mice. The specificity of the antibody was determined by Western blot analysis. All results suggested that Mimi-HSP90 was involved in thermal stress and immune response in miiuy croaker.
Journal: Fish & Shellfish Immunology - Volume 35, Issue 2, August 2013, Pages 429–437