کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2431959 1106775 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An IRF-3 homolog that is up-regulated by DNA virus and poly I:C in turbot, Scophthalmus maximus
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
An IRF-3 homolog that is up-regulated by DNA virus and poly I:C in turbot, Scophthalmus maximus
چکیده انگلیسی

In this study, we described the structure, mRNA tissue distribution and regulation of an IRF-3 gene from turbot, Scophthalmus maximus (SmIRF-3). The gene sequence of SmIRF-3 is 6077 bp long, composed of 11 exons and 10 introns similar to known IRF-3 genes of fish, and encodes a peptide of 466 amino acids. The deduced protein sequence shares the highest identity of 56.0–81.2% with fish IRF-3 and possesses a DNA-binding domain (DBD), an IRF association domain (IAD) and a serine-rich domain (SRD) known to be important for the functions of IRF-3 in vertebrates. Phylogenetic analysis grouped SmIRF-3 with other IRF3s of vertebrates. SmIRF-3 transcripts were detectable in limited tissue types of healthy fish, with higher expression observed in head, kidney, spleen and kidney,. The SmIRF-3 was transcriptionally up-regulated by turbot reddish body iridovirus (TRBIV) and polyinosinic:polycytidylic acid (poly I:C) in the head kidney, spleen and gills, with showing a two wave induced expression during a 7-day time course in all cases. The highest inducibility and the likely earliest increase of SmIRF-3 expression were observed in the spleen, and poly I:C was a stronger inducer. In addition, the maximal expression level of SmIRF-3 arose prior to that of the Mx in all the cases.


► The gene sequence of an IRF-3 homolog in turtbot was cloned.
► Turbot IRF-3 transcripts were more abundant in immune-relative organs.
► Turbot IRF-3 was up-regulated by turbot reddish body iridovirus and poly I:C.
► The highest inducibility and the earliest increase were observed in the spleen.
► The maximal level of turbot IRF-3 expression arose prior to that of the Mx.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 31, Issue 6, December 2011, Pages 1224–1231
نویسندگان
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