Immune role of MrNFκBI-α, an IκB family member characterized in prawn M. rosenbergii

NF kappa B inhibitor alpha (MrNFκBI-α) was sequenced from a freshwater prawn Macrobrachium rosenbergii. The MrNFκBI-α protein contains a long ankyrin repeat region circular domain between 193 and 413 along with its 6 repeats (ankyrin repeat 1,2,3,4,5 and 6). An IκB degradation motif and a putative PEST motif is present at 37–64 and 418–471 of the N- and C-terminal regions of MrNFκBI-α respectively. The gene expressions of MrNFκBI-α in healthy and infectious hematopoietic and hypodermal necrosis virus (IHHNV), poly I:C, Aeromonas hydrophila and Enterococcus faecium injected M. rosenbergii were examined using quantitative real time PCR. The MrNFκBI-α is expressed in all the tissues taken for examination and the highest is observed in hemocytes. The MrNFκBI-α gene expression is strongly up-regulated in hemocytes of prawn after IHHNV, poly I:C, A. hydrophila and E. faecium infection. This result indicates an important role of MrNFκBI-α in M. rosenbergii immune system. This, however, remains to be verified by further studies.

► MrNFκBI-α protein contains six ankyrin repeats between 193 and 413.
► An IκB degradation motif is present at the N-terminal of MrNFκBI-α.
► In C-terminal, a putative PEST motif is present at 418–471.
► MrNFκBI-α is expressed in all the tissues and the highest at hemocytes.
► Gene expression is up-regulated after viral and bacterial immune injection.