کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2432188 1106784 2012 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fish cell cultures as in vitro models of pro-inflammatory responses elicited by immunostimulants
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Fish cell cultures as in vitro models of pro-inflammatory responses elicited by immunostimulants
چکیده انگلیسی

We have tested the elicitation of innate defence-related responses in two stromal cell lines derived from the spleen (trout splenic stroma, TSS) and the pronephros (trout pronephric stroma-2, TPS-2) of rainbow trout (Oncorhynchus mykiss) after they were exposed to different concentrations of lipopolysaccharide (LPS), levamisole, or polyinosinic polycytidylic acid (poly-I:C). For comparison, cultures of rainbow trout head kidney macrophages were also included in the study, and the effect of the immunostimulants on the phagocytic activity, the intracellular and extracellular reactive oxygen species and nitric oxide production were assayed. Although the responses varied depending upon the concentration of the immunostimulants and the particular cell line, our results demonstrate that those activities were enhanced in the TSS and TPS-2 cell lines after exposure to any of the immunostimulants. These results indicate that the stromal cells of the main lympho-haemopoietic organs of O. mykiss develop innate defence responses, which are enhanced by well-known immunostimulants. In addition, such enhancement of the defence responses in the TSS and TPS-2 cell lines could be also elicited when they were exposed to conditioned supernatants from levamisole- or poly I:C-stimulated HK macrophage cultures, thus demonstrating that the haemopoietic stromal cells respond to macrophage-derived factors. Moreover, we demonstrate that the stromal cell lines constitutively expressed the Toll-like receptors TLR3, TLR5 and TLR9 genes. The results are discussed considering the role of the lympho-haemopoietic stromal cells in the innate immune responses, and the possibility of using histiotypic cell cultures of non-leucocyte cells of the haemopoietic organs to develop in vitro methods to select new immunostimulant candidates for aquaculture.


► We tested the effects of immunostimulants on trout haemopoietic stromal cell lines.
► Rainbow trout pronephric (TPS-2) and splenic (TSS) stromal cell lines were used.
► LPS, levamisole and poly I:C were used as immunostimulants.
► Immunostimulants boosted phagocytosis, ROS – NO production and bactericidal activity.
► Conditioned supernatants from macrophage cultures also stimulated TPS-2 and TSS cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 33, Issue 2, August 2012, Pages 389–400
نویسندگان
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