A piscidin-like antimicrobial peptide from the icefish Chionodraco hamatus (Perciformes: Channichthyidae): Molecular characterization, localization and bactericidal activity

Antimicrobial peptides (AMPs) are considered one of the most ancient components of the innate immune system. They are able to exert their protection activity against a variety of microorganisms, and are widely distributed in both vertebrates and invertebrates. In this paper we focused on an AMP identified in the Antarctic teleost Chionodraco hamatus, an icefish species. The cDNA sequence of the AMP, named chionodracine, is comprised of 515 bp and translates for a putative protein precursor of 80 amino acids, with a signal peptide of 22 amino acids. The structural features evidenced in the primary sequence of chionodracine lead to the inclusion of the peptide in the antimicrobial family of piscidins. The analysis by real-time PCR of the basal gene transcripts of chionodracine in different icefish tissues showed that the highest expression was found in gills, followed by head kidney. The chionodracine expression levels in head kidney leukocytes were up-regulated in vitro both by LPS and poly I:C, and in vivo by LPS. A putative chionodracine mature peptide was synthesized and employed to obtain a polyclonal antiserum, which was used in immunohistochemistry of gills sections and revealed a significant positivity associated with mast cells. The bactericidal activity of the peptide was investigated and found significant against Antarctic psychrophilic bacteria strains (Psychrobacter sp. TAD1 and TA144), the Gram-positive Bacillus cereus, and at a lesser extent against the Gram-negative Escherichia coli. Interestingly, the haemolytic activity of chionodracine was tested in vitro on human erythrocytes and no significant lysis occurred until peptide concentration of 50 μM.

► We have identified an antimicrobial peptide from the icefish Chionodraco hamatus.
► The structural features of the peptide led to its inclusion in the piscidins family.
► The regulation of its gene expression has been investigated both in vitro and in vivo.
► The chionodracine is present in the mast cells of the gills.
► The bactericidal activity of chionodracine has been investigated.