کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2432741 1106803 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Subcellular components of probiotics Kocuria SM1 and Rhodococcus SM2 induce protective immunity in rainbow trout (Oncorhynchus mykiss, Walbaum) against Vibrio anguillarum
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Subcellular components of probiotics Kocuria SM1 and Rhodococcus SM2 induce protective immunity in rainbow trout (Oncorhynchus mykiss, Walbaum) against Vibrio anguillarum
چکیده انگلیسی

The efficacy of cellular components of probiotics Kocuria SM1 and Rhodococcus SM2 to protect rainbow trout (Oncorhynchus mykiss, Walbaum) against vibriosis was assessed. Groups of fish (average weight = 10–15 g) were immunized intraperitoneally (i.p.) with 0.1 ml of subcellular materials, i.e., 0.2 ± 0.05 mg protein per fish, comprising extracellular proteins (ECPs), cell wall proteins (CWPs) and whole cell proteins (WCPs) of SM1 and SM2, respectively, or with 0.1 ml of phosphate-buffered saline (PBS) to serve as the control. Seven days after administration, fish from each group were challenged i.p. with 0.1 ml of a suspension in PBS of 3 × 105 cells ml−1 per fish of Vibrio anguillarum. Use of CWPs and WCPs demonstrated significantly (P < 0.05) better protection against V. anguillarum insofar as mortalities were reduced to 11–17% [relative percent survival (RPS) = 80–87%], although ECPs fared less well (mortalities = 33–38%; RPS = 56–62%; P > 0.05), compared to 86% mortalities of the controls. The mode of action reflected activation of innate immune factors by CWPs and WCPs, demonstrating significantly (P < 0.05) increased expression of respiratory burst (optical density; OD550nm) from 0.039 to 0.043–0.045, peroxidase (OD550nm) from 0.26 to 0.37–0.55, and bacterial killing activities (i.e., percentage of surviving bacteria reduced from 79% to 56–57% for SM2). Moreover, an elevation of leucocyte number (from 1.93% to 1.98–2.93%; P > 0.05) and immunoglubolin level (from 27 mg ml−1 to 28.5–33 mg ml−1; P > 0.05) were observed with the experimental groups. These results indicate that cell components of the probiotics stimulate an immune response.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 30, Issue 1, January 2011, Pages 347–353
نویسندگان
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