کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2432951 1106811 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Genomic organization and mRNA expression of manganese superoxide dismutase (Mn-SOD) from Hemibarbus mylodon (Teleostei, Cypriniformes)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Genomic organization and mRNA expression of manganese superoxide dismutase (Mn-SOD) from Hemibarbus mylodon (Teleostei, Cypriniformes)
چکیده انگلیسی

The genomic structure of manganese superoxide dismutase (Mn-SOD) was characterized in Hemibarbus mylodon (Teleostei, Cypriniformes), an endangered freshwater fish species, and changes in Mn-SOD mRNA expression in response to various stimuli, such as challenge with lipopolysaccharide (LPS), bacteria, or heavy metals, were examined. H. mylodon Mn-SOD possesses well-conserved features of vertebrate Mn-SODs, including its homologous quinquepartite gene structure (five exons interrupted by four introns) and high sequence identity with vertebrate orthologs in coding regions, including a typical eukaryotic Mn-SOD signature. The 5′-regulatory upstream region of the H. mylodon Mn-SOD gene lacked a canonical TATA sequence; however, it displayed various transcription factor binding elements that may be involved in immune and stress responses, as well as the modulation of enzymes that mediate the metabolism of metals and other xenobiotics. Bioinformatic analysis of Mn-SOD genes from fish and mammalian genome databases revealed chromosomal synteny of the Mn-SOD locus in the vertebrate lineage. Mn-SOD transcripts were ubiquitously detected in diverse tissues with variable levels of basal expression using an RT-PCR assay. Mn-SOD expression in the liver and kidney were significantly modulated by injection of LPS (1, 5, or 10 μg g−1 body weight), Edwardsiella tarda challenge (5 × 103 or 5 × 105 cells/fish), and heavy metal exposure (Cd, Cu, or Zn at 5 μM). Results from this study suggest that Mn-SOD plays an important role in the host defence against oxidative damage caused by infection-mediated inflammation and/or toxicant related stress in this species.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 27, Issue 4, October 2009, Pages 571–576
نویسندگان
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