کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2433616 1106841 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular cloning and expression analysis of sea bass (Dicentrarchus labrax L.) tumor necrosis factor-α (TNF-α)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Molecular cloning and expression analysis of sea bass (Dicentrarchus labrax L.) tumor necrosis factor-α (TNF-α)
چکیده انگلیسی

In the search for pro-inflammatory genes in sea bass a TNF-α gene was cloned and sequenced. The sea bass TNF-α (sbTNF-α) putative protein conserves the TNF-α family signature, as well as the two cysteines usually involved in the formation of a disulfide bond. The mouse TNF-α Thr-Leu cleavage sequence and a potential transmembrane domain were also found, suggesting that sbTNF-α exists as two forms: a ∼28 kDa membrane-bound form and a ∼18.4 kDa soluble protein. The single copy sbTNF-α gene contains a four exon-three intron structure similar to other known TNF-α genes. Homology modeling of sbTNF-α is compatible with the trimeric quaternary architecture of its mammalian counterparts. SbTNF-α is constitutively expressed in several unstimulated tissues, and was not up-regulated in the spleen and head-kidney, in response to UV-killed Photobacterium damselae subsp. piscicida. However, an increase of sbTNF-α expression was detected in the head-kidney during an experimental infection using the same pathogen.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 23, Issue 3, September 2007, Pages 701–710
نویسندگان
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