کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2440951 | 1108129 | 2006 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Conjugated Linoleic Acid Reduces Phorbol Ester-Induced Prostaglandin F2α Production by Bovine Endometrial Cells1
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
Recent interest in conjugated linoleic acid (CLA) research stems from the well-documented anticarcinogenic, antiatherogenic, antidiabetic, and antiobesity properties of CLA in animal models. The objective of this study was to examine the effects of 2 CLA isomers (cis-9,trans-11 and trans-10,cis-12) on phorbol 12,13-dibutyrate (PDBu)-induced PGF2α production in cultured bovine endometrial (BEND) cells. Confluent BEND cells were incubated in the absence (control) or presence of 100 μM each of linoleic acid, cis-9,trans-11 CLA, or trans-10,cis-12 CLA for 24 h. After incubation, cells were rinsed and then stimulated with PDBu (100 ng/mL) for 6 h. Compared with untreated cells, PDBu stimulated PGF2α secretion (+25-fold) within 6 h. The increases in PGF2α secretion were paralleled by signifi-cant induction of prostaglandin endoperoxide synthase-2 (PGHS-2) mRNA (+63-fold) and protein (+1.6-fold) expression. In spite of stimulatory effects on PGHS-2 and peroxisome proliferator-activated receptor δ (PPARδ) mRNA responses, CLA greatly decreased PGF2α production by PDBu-stimulated BEND cells. There was no evidence for PDBu or CLA modulation of PPARδ protein synthesis in cultured BEND cells. Results indicated that CLA modulation of PGF2α production by BEND cells was not mediated through PGHS-2 or PPARδ gene repression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dairy Science - Volume 89, Issue 10, October 2006, Pages 3826-3832
Journal: Journal of Dairy Science - Volume 89, Issue 10, October 2006, Pages 3826-3832
نویسندگان
C. Rodriguez-Sallaberry, C. Caldari-Torres, E.S. Greene, L. Badinga,