کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2450907 1109665 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Meat species identification and Halal authentication analysis using mitochondrial DNA
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Meat species identification and Halal authentication analysis using mitochondrial DNA
چکیده انگلیسی

A method utilizing PCR-restriction fragment length polymorphism (RFLP) in the mitochondrial genes was developed for beef (Bos taurus), pork (Sus scrofa), buffalo (Bubalus bubali), quail (Coturnix coturnix), chicken (Gallus gallus), goat (Capra hircus), rabbit (Oryctolagus cuniculus) species identification and Halal authentication. PCR products of 359-bp were successfully obtained from the cyt b gene of these six meats. AluI, BsaJI, RsaI, MseI, and BstUI enzymes were identified as potential restriction endonucleases to differentiate the meats. The genetic differences within the cyt b gene among the meat were successfully confirmed by PCR-RFLP. A reliable typing scheme of species which revealed the genetic differences among the species was developed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Meat Science - Volume 83, Issue 1, September 2009, Pages 57–61
نویسندگان
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