کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2454734 1554313 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular characterization of RNA binding motif protein 3 (RBM3) gene from Pashmina goat
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Molecular characterization of RNA binding motif protein 3 (RBM3) gene from Pashmina goat
چکیده انگلیسی


• Full length RBM3 gene from Pashmina goat was fished out, the ORF of which was found to be 273 bp in length.
• Phylogenetic analysis of Pashmina RBM3 gene revealed that it is closely related to Bos Taurus RBM3.
• qRT-PCR analysis revealed that RBM3 gene expression was significantly higher at moderate hypothermia than at deep hypothermia.
• In-silico comparative 3D model of the Pashmina RBM3 was generated which contained 2 α-helices, 7 coils and 4 β-sheets.

Pashmina goat inhabits the high altitude cold arid desert of Ladakh, India. This goat is known for its finest and costliest under fiber. Though the under fiber may be a part of its complex thermoregulation mechanism, the genetics of its adaptability under cold conditions is not known. As an attempt to understand its adaptive genetics, and the role of RNA-binding proteins at the cellular response, this study was conducted to characterize the RBM3 gene in Pashmina goat and its expression during hypothermia. The ORF of Pashmina RBM3 gene was 273 bp. Phylogenetic analysis revealed that Pashmina RBM3 is closely related to Bos taurus RBM3. Pashmina RBM3 was characterized by comparative modeling studies. The final 3-D model contained two α-helices and four β-sheets. qRT-PCR data showed that Pashmina RBM3 gene expression was significantly higher (P < 0.05) at moderate (30 °C) hypothermic stress conditions as compared with deep (15 °C) hypothermia.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Veterinary Science - Volume 98, February 2015, Pages 51–58
نویسندگان
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