کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2454880 1554312 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The effect of mitochondrial ATP-sensitive potassium channels on apoptosis of chick embryo cecal cells by Eimeria tenella
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
The effect of mitochondrial ATP-sensitive potassium channels on apoptosis of chick embryo cecal cells by Eimeria tenella
چکیده انگلیسی


• We applied primary chick embryo cecal cells as host cells in vitro experiments.
• E. tenella-infected cells after diazoxide treatment underwent less apoptosis.
• Activities of caspases 9 and 3 were less activated in diazoxide-treated cells.
• MitoKATP channels play a key role in protecting cells from apoptosis by E. tenella.

The objective of this study was to investigate the effect of mitochondrial ATP-sensitive potassium (mitoKATP) channels on apoptosis induced by Eimeria tenella. At 24, 48, 72, 96 and 120 h after Eimeria tenella infection, TUNEL assays and translation of phosphatidyl serines to the host cell plasma membrane surface showed that diazoxide-treated chick embryo cecal cells underwent less apoptosis (P < 0.05), while light microscopy showed that infection rates of treated cells were higher (P < 0.01) than untreated cells. Caspase 9 and caspase 3 of infected cells were activated less (P < 0.01) in diazoxide-treated cells than untreated cells. These results indicate that opening mitoKATP channels can protect chick embryo cecal cells from mitochondria-dependent apoptosis induced by Eimeria tenella by inhibiting activations of caspase 9 and caspase 3.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Veterinary Science - Volume 99, April 2015, Pages 188–195
نویسندگان
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