کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2455126 | 1110506 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Reprogramming of fetal cells by avian EE for generation of pluripotent stem cell like cells in caprine
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Reprogramming of fetal cells by avian EE for generation of pluripotent stem cell like cells in caprine Reprogramming of fetal cells by avian EE for generation of pluripotent stem cell like cells in caprine](/preview/png/2455126.png)
چکیده انگلیسی
The present work was carried out to study the ability of avian “Extract Egg” (EE) for reprogramming caprine fetal cells. The isolated caprine fetal cells were cultured in stem cell media supplemented with different percentages of either EE or FBS. The results indicated that the supplementation of 2-4% EE formed lesser but larger size stem cell like cell colonies as compared to 6% or 10% EE. The expression of pluripotent genes were comparatively higher in colonies developed in 2% or 4% as compared to 6% or 10% EE. Further, immunocytochemistry revealed that the colonies developed in all percentage of EE expressed pluripotent markers like Oct4, Nanog, TRA-1-60 and TRA-1-81. Our findings indicated that avian EE has the potentiality to reprogram caprine fetal cells into embryonic state which may help in generation of pluripotent stem cells without using viral vector.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Veterinary Science - Volume 95, Issue 2, October 2013, Pages 638-643
Journal: Research in Veterinary Science - Volume 95, Issue 2, October 2013, Pages 638-643
نویسندگان
Parul Bharadwaj, Kuldeep Kumar, Renu Singh, Gopal Puri, T. Yasotha, Manish Kumar, Sanjeev Bhure, B.C. Das, M. Sarkar, Sadhan Bag,