کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2456691 1554356 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of low molecular weight cryoprotectants on the post-thaw ram sperm quality and fertilizing ability
ترجمه فارسی عنوان
اثرات ضد سرطان پروستات کم مولکولی بر کیفیت اسپرم پس از خنک کننده و توانایی باروری
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی


• Low molecular weight cryoprotectants for ram semen cryopreservation.
• Semen collection, cryopreservation and in vitro fertilization.
• DMSO had a deleterious effect on the post thaw ram sperm parameters and embryonic development.
• Ethylene glycol and 1,2 propanediol succesfully protected acrosomal and DNA integrities.
• Glycerol provided a better cryoprotection to sperm motility and plasma membrane integrity.

The aim of the current study was to compare the cryoprotective effects of low molecular weight cryoprotectants 6% DMSO, 6% ethylene glycol, 6% 1,2 propanediol and 6% glycerol on the efficiency of ram semen cryopreservation and to test the in vitro fertilizing ability of frozen–thawed ram semen. Ejaculates with a thick consistency, rapid wave motion (3–5), and >75% initial motility were pooled. Sperm were diluted to a final concentration of 1/5 (semen/extender) in 6% glycerol, 6% DMSO, 6% ethylene glycol and 6% 1,2 propanediol using a two-step dilution method. Two hours equilibrated semen was frozen in 0.25-ml straws. As expected, the results of the current study showed that motility, HOST values and the rates of defective acrosomes in sperm were negatively affected by the cryopreservation procedure (P < 0.01). In conclusion, 6% DMSO had a deleterious effect on the post thaw ram sperm parameters and embryonic development. Ethylene glycol and 1,2 propanediol successfully protected acrosomal and DNA integrities. Glycerol provided a better cryoprotection to sperm motility and plasma membrane integrity. The results of in vitro fertilization, as assessed by the rate of blastocyst formation, in glycerol group were similar to 1,2 propanediol group.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Small Ruminant Research - Volume 136, March 2016, Pages 59–64
نویسندگان
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