Molecular, biological, and morphometric comparisons between different geographical populations of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae)

• Different geographic populations of Rhipicephalus sanguineus sensu lato were analyzed.
• These populations were compared by molecular, biological, and morphometric methods.
• Comparisons using 12S and 16S rDNA sequences showed two distinct clades.
• Morphometric and biological analysis showed good separation between the two clades.
• These results confirm the existence of two species under the name “R. sanguineus”.

In this study, different geographical populations of Rhipicephalus sanguineus sensu lato were compared by molecular, biological, and morphometric methods. Phylogenetic trees were constructed using 12S and 16S rDNA sequences and showed two distinct clades: one composed of ticks from Brazil (Jaboticabal, SP), Cuba (Havana) Thailand (Bangkok) and the so-called “tropical strain” ticks. The second clade was composed of ticks from Spain (Zaragoza), Argentina (Rafaela, Santa Fe) and the so-called “temperate strain” ticks. Morphometric analysis showed good separation between females of the two clades and within the temperate clade. Males also exhibited separation between the two clades, but with some overlap. Multiple biological parameters revealed differences between the two clades, especially the weight of the engorged female. These results confirm the existence of at least two species under the name “R. sanguineus”.

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