کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2471023 | 1555748 | 2009 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Prolonged resilience of Tritrichomonas foetus in cat faeces at ambient temperature Prolonged resilience of Tritrichomonas foetus in cat faeces at ambient temperature](/preview/png/2471023.png)
We have investigated the diagnostic utility of culture methods for Tritrichomonas foetus from cat faeces and the influence of faecal storage on the sensitivity of two different culture systems, InPouch TF-Feline (Bio-Med Diagnostics) and Modified Diamond's Medium. Normally formed cat faeces were spiked using a 10-fold serial dilution of 2 × 102 to 2 × 105T. foetus per gram of faeces prior to storage at either room temperature or under refrigeration. At different time points a 20 mg sample of spiked faeces was inoculated into each of the culture systems which were evaluated daily for up to 7 days and on day 11. Successfully cultured T. foetus 24 h following faecal spiking and storage at room temperature demonstrates that the environmental resilience of the organism is greater than previously anticipated. At a conservative detection limit of 2 × 103 organisms per gram of faeces the accumulative sensitivity of each culture method was found to be 83% and 100% for the InPouch TF-Feline and Modified Diamond's Medium respectively over a 6 h period. This research demonstrates that voided faeces kept at room temperature for up to 6 h represent suitable material for diagnostic investigation. The resilience of T. foetus suggests that transmission is not limited to close contact between cats.
Journal: Veterinary Parasitology - Volume 166, Issues 1–2, 3 December 2009, Pages 60–65