کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2471964 1555782 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Viability and infectivity of Trichinella spiralis muscle larvae in frozen horse tissue
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Viability and infectivity of Trichinella spiralis muscle larvae in frozen horse tissue
چکیده انگلیسی

Many aspects of the biology and epidemiology of Trichinella infection in the horse are poorly understood, including survival of Trichinella spp in horse muscle. In this study, we have assessed the freeze tolerance of T. spiralis in horse meat stored at 5, −5, and −18 °C for 1 day to 24 weeks. Results demonstrate a steady reduction in the number of live ML recovered from the cold stored meat samples. On Day 1, recovery of live larvae had been reduced by 18.6%, 50.1%, and 37.2%, and by 4 weeks, recovery of larvae had been reduced by 65.4%, 66.5%, and 96.2% in samples stored at 5, −5, and −18 °C, respectively. Infectivity results (measured as reproductive capacity index (RCI)) from mice inoculated with larvae recovered from non-frozen meat samples at day 0 was 23.5. Following storage at −18 °C for one and two days, the RCIs were 2.09 and 0.99, respectively. Small numbers of infective larvae were still present in meat samples stored at −18 °C for 4 weeks. The RCI of ML recovered from meat samples stored at −5 °C was 14.99 and 6.36 at 2 weeks and 4 weeks respectively; the RCI of samples stored at 5 °C was 23.1 at 8 weeks, and fell rapidly thereafter (12 week RCI 1.33; 0 at 24 weeks). These data demonstrate that infective T. spiralis, a non-freeze tolerant species, can survive for at least 4 weeks in horse tissue frozen at −5 or −18 °C, and that the numbers of infective larvae decrease substantially by day 2 at −18 °C and by week 4 at −5 °C.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Parasitology - Volume 146, Issues 1–2, 15 May 2007, Pages 102–106
نویسندگان
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