کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2471994 1555783 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The main proteinases in Dermatobia hominis second and third instars larvae are serine-proteinases
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
The main proteinases in Dermatobia hominis second and third instars larvae are serine-proteinases
چکیده انگلیسی

We performed a combination of proteinase assay, either in solution or immobilized in sodium dodecyl sulfate-polyacrylamide gel copolymerized with gelatin, to detect and quantify proteinases of Dermatobia hominis second (L2) and third (L3) instar larvae. In the quantitative assay, we examined proteinase activity by hydrolysis of a panel of peptide bonds specific for the main proteinase classes. We verified that the pGlu-Phe-Leu p-nitroanilide substrate was hydrolyzed by crude extracts of L2 (3.0 ± 0.2 nmol h−1 mg of protein−1) and L3 (7.7 ± 0.1 nmol h−1 mg of protein−1) and that both activities were partially inhibited by trans-epoxysuccinyl-l-leucylamido-(4-guanidino)butane, 15% and 3%, respectively. Also, we demonstrated that the Nα-p-Tosyl-l-Arg methyl ester substrate was hydrolyzed by crude extracts of L2 (117 ± 24 nmol h−1 mg of protein−1) and L3 (111 ± 10 nmol h−1 mg of protein−1), suggesting a predominance of esterase activity in the crude larval preparation. Interestingly, the specific activity of serine-proteinases was totally inhibited by phenylmethylsulphonyl fluoride in the L3 crude extract, while only 10% of this enzyme class activity was inhibited in the L2 crude extract. The results of the qualitative assays with substrate gels suggested that L2 and L3 larvae express serine-proteinases with similar (13 and 22 kDa) and distinct (50 kDa in L2 and 30 kDa in L3) relative molecular masses. These findings contribute to the biochemical characterization of D. hominis L2 and L3 larvae.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Parasitology - Volume 145, Issues 3–4, 30 April 2007, Pages 326–331
نویسندگان
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