کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2480495 1556187 2014 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Encapsulation of antigen-loaded silica nanoparticles into microparticles for intradermal powder injection
ترجمه فارسی عنوان
کپسول سازی نانوذرات سیلیکا آنتیژن لخته شده به میکروپارسیت برای تزریق پودر داخل بینی
کلمات کلیدی
نانوذرات، ذرات کوچک، سیلیسیوم مزوپور، واکسیناسیون، سیستم نانو در میکروپارسیت
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی اکتشاف دارویی
چکیده انگلیسی

Epidermal powder immunisation (EPI) is being investigated as a promising needle-free delivery methods for vaccination. The objective of this work was to prepare a nanoparticles-in-microparticles (nano-in-micro) system, integrating the advantages of nanoparticles and microparticles into one vaccine delivery system for epidermal powder immunisation. Cationic mesoporous silica nanoparticles (MSNP-NH2) were prepared and loaded with ovalbumin as a model antigen. Loading was driven by electrostatic interactions. Ovalbumin-loaded silica nanoparticles were subsequently formulated into sugar-based microparticles by spray-freeze-drying. The obtained microparticles meet the size requirement for EPI. Confocal microscopy was used to demonstrate that the nanoparticles are homogeneously distributed in the microparticles. Furthermore, the silica nanoparticles in the dry microparticles can be re-dispersed in aqueous solution showing no aggregation. The recovered ovalbumin shows integrity compared to native ovalbumin. The present nano-in-micro system allows (1) nanoparticles to be immobilized and finely distributed in microparticles, (2) microparticle formation and (3) re-dispersion of nanoparticles without subsequent aggregation. The nanoparticles inside microparticles can (1) adsorb proteins to cationic shell/surface voids in spray-dried products without detriment to ovalbumin stability, (2) deliver antigens in nano-sized modes to allow recognition by the immune system.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmaceutical Sciences - Volume 63, 15 October 2014, Pages 154–166
نویسندگان
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