کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2482392 1556265 2008 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of an in situ forming PLGA drug delivery system: I. Characterization of a non-aqueous protein precipitation
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی اکتشاف دارویی
پیش نمایش صفحه اول مقاله
Development of an in situ forming PLGA drug delivery system: I. Characterization of a non-aqueous protein precipitation
چکیده انگلیسی

The incorporation of the model protein hen egg white lysozyme into liquid in situ forming poly(lactide-co-glycolide) (PLGA) implant or microparticle formulations was investigated. Ternary solvent blends of dimethyl sulfoxide (DMSO), ethyl acetate and water were used to adjust the protein solubility in order to facilitate the incorporation of either dispersed or dissolved protein into the polymer solution. Lysozyme formed large gel particles when dispersed directly in the polymer solution. These formulations had a pronounced initial release. Non-aqueous precipitation of lysozyme from solutions in DMSO with ethyl acetate led to a reversible aggregation without loss in biological activity. Lysozyme could be incorporated in a finely dispersed state through an in situ precipitation by non-solvent or polymer addition. Non-aqueous precipitation could thus be utilized to manufacture biodegradable in situ forming drug delivery systems containing homogeneously distributed and bioactive protein.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmaceutical Sciences - Volume 35, Issue 4, 15 November 2008, Pages 283–292
نویسندگان
, ,