کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2482396 | 1556265 | 2008 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular cloning and functional characterization of cynomolgus monkey multidrug resistance-associated protein 2 (MRP2)
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کلمات کلیدی
ABCMRPMrp2LTC4DNP-SGCanalicular membrane vesiclesABC transporter - ABC حمل کنندهInterspecies difference - تفاوت بین گونه ایCMV - سیتومگالوویروسATPase activity - فعالیت ATPaseLeukotriene C4 - لوکوترین C4cynomolgus monkey - میمون cynomolgusmultidrug resistance-associated protein - پروتئین مرتبط با مقاومت چند داروییATP-binding cassette - کیت اتصال به ATP
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular cloning and functional characterization of cynomolgus monkey multidrug resistance-associated protein 2 (MRP2) Molecular cloning and functional characterization of cynomolgus monkey multidrug resistance-associated protein 2 (MRP2)](/preview/png/2482396.png)
چکیده انگلیسی
The monkey is an important experimental model in the pharmacological evaluation of new drugs. We isolated monkey multidrug resistance-associated protein 2 (MRP2) cDNA to examine expression profiles among various tissues and measured ATPase activity to assess substrate specificity. The amino acid sequence encoded by monkey MRP2 cDNA was very similar (96% identity) to the reported human MRP2 cDNA (GenBank accession no. NM_000392). The tissue distribution of MRP2 in monkeys was partially different from that in humans. We found relatively high expression of MRP2 in the monkey kidney and small intestine using Northern blotting. Substrate specificity was compared between human and monkey MRP2. The affinity of 17β-estradiol 17-(β-d-glucuronide), methotrexate, vinblastine, and probenecid to monkey MRP2 was higher than that to human MRP2. Functional and expression differences between human and monkey MRP2 should be incorporated into the evaluation of candidate drugs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmaceutical Sciences - Volume 35, Issue 4, 15 November 2008, Pages 326-334
Journal: European Journal of Pharmaceutical Sciences - Volume 35, Issue 4, 15 November 2008, Pages 326-334
نویسندگان
Masa Yasunaga, Masaaki Takemura, Kyoko Fujita, Hikaru Yabuuchi, Morimasa Wada,