کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2540243 1559757 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Loxoprofen sodium induces the production of complement C5a in human serum
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Loxoprofen sodium induces the production of complement C5a in human serum
چکیده انگلیسی


• The 24 h culture of blood with loxoprofen induced basophil activation
• Loxoprofen induced a basophil activator in serum on the 24 h incubation.
• The activator induced by loxoprofen is a Complement C5a.

Basophil activation test (BAT) is an in vitro allergy test that is useful to identify allergens that cause IgE-dependent allergies. The test has been used to detect not only food allergies and allergies caused by environmental factors but also to detect drug hypersensitivity, which has been known to include IgE-independent reactions. In our preliminary studies in which BAT was applied to detect hypersensitivity of loxoprofen, a non-steroidal anti-inflammatory drug (NSAID), conventional BAT with incubation for 30 min did not show basophil activation by means of increased CD203c expression. In this study, we extended the incubation time to 24 h on the basis of the hypothesis that loxoprofen indirectly activates basophils. Basophils from healthy control donors as well as allergic patients showed up-regulation of CD203c after incubation with loxoprofen for 24 h. Activation was induced using loxoprofen-treated serum. Proteomic and pharmacologic analyses revealed that serum incubation with loxoprofen generated an active complement component C5a, which induced CD203c expression via binding to the C5a receptor on basophils. Because C3a production was also detected after incubation for 24 h, loxoprofen is likely to stimulate the complement classical pathway. Our findings suggest that the complement activation is involved in drug hypersensitivity and the suppression of this activation may contribute to the elimination of false positive of BAT for drug allergies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 33, April 2016, Pages 55–62
نویسندگان
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