کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2540306 1559755 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Optimization of a methamphetamine conjugate vaccine for antibody production in mice
ترجمه فارسی عنوان
بهینه سازی یک واکسن متامفتامین مزدوج برای تولید آنتی بادی در موش
کلمات کلیدی
GLA، glucopyranosyl لیپید A؛ ICKLH، مونومرهای ایمونوسیانین KLH؛ IM، عضلانی KLH، کلم هول لیفتوم همایانین؛ LPS، lipopolysaccharide؛ MCV، واکسن متشکل از METH؛ مت، (+) - متامفتامین؛ SMO9، Hapten-like METH (S) -N- (2- (مری کپتو اتیل) -6- (3- (2- (× äŊŊĐĐ Č ✓
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
چکیده انگلیسی


• METH-conjugate vaccine produces high affinity antibody response in mice.
• The range of hapten densities tested did not greatly affect the antibody response.
• GLA-SE adjuvant produces higher antibody levels than other tested adjuvants.
• Adjuvants like GLA-SE may be a key to effective human substance abuse vaccines.

There are still no approved medications for treating patients who abuse methamphetamine. Active vaccines for treating abuse of nicotine and cocaine are in clinical studies, but have not proven effective seemingly due to inadequate anti-drug antibody production. The current studies aimed to optimize the composition, adjuvant and route of administration of a methamphetamine conjugate vaccine, ICKLH-SMO9, in mice with the goal of generating significantly higher antibody levels. A range of hapten epitope densities were compared, as were the adjuvants Alhydrogel and a new Toll-like receptor 4 (TLR4) agonist called GLA-SE. While methamphetamine hapten density did not strongly affect the antibody response, the adjuvant did. Glucopyranosyl lipid A in a stable oil-in-water emulsion (GLA-SE) produced much higher levels of antibody in response to immunization compared with Alhydrogel; immunization with GLA-SE also produced antibodies with higher affinities for methamphetamine. GLA-SE has been used in human studies of vaccines for influenza among others and like some other clinical TLR4 agonists, it is safe and elicits a strong immune response. GLA-SE adjuvanted vaccines are typically administered by intramuscular injection and this also proved effective in these mouse studies. Clinical studies of the ICKLH-SMO9 methamphetamine vaccine adjuvanted with GLA-SE have the potential for demonstrating efficacy by generating much higher levels of antibody than substance abuse vaccines that have unsuccessfully used aluminum-based adjuvants.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 35, June 2016, Pages 137–141
نویسندگان
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