کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2540581 1122600 2015 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Scavenger receptor for lipoteichoic acid is involved in the potent ability of Lactobacillus plantarum strain L-137 to stimulate production of interleukin-12p40
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Scavenger receptor for lipoteichoic acid is involved in the potent ability of Lactobacillus plantarum strain L-137 to stimulate production of interleukin-12p40
چکیده انگلیسی


• Heat-killed Lactobacillus plantarum strain L-137 (HK L-137) is a potent inducer of interleukin(IL)-12.
• HK L-137 exposes more lipoteichoic acid(LTA) on its cell surface than other strains.
• Phagocytosis was correlated with IL-12P40 production by murine splenic adherent cells.
• Scavenger receptor A(SR-A) for LTA of HK L-137 is responsible for higher efficiency of phagocytosis of HK L-137.
• SR-A-mediated phagocytosis is responsible for the potent ability of HK L-137 to induce IL-12p40.

Heat-killed Lactobacillus plantarum strain L-137 (HK L-137) is a more potent inducer of interleukin (IL)-12 than other heat-killed Lactobacillus strains. To elucidate the mechanism involved in this IL-12p40 induction, we compared HK L-137 with heat-killed L. plantarum strain JCM1149 (HK JCM1149) by nuclear magnetic resonance and mass spectrometry. Results showed that HK L-137 contained lipoteichoic acid (LTA) with a chemical structure similar to that of JCM1149, except for a lower degree of glucosyl substitution in the poly(glycerol phosphate) backbone. Lysozyme sensitivity and electrophoretic moiety analysis revealed that HK L-137 exposed more LTA on its cell surface than HK JCM1149. Phagocytosis of HK L-137 by splenic adherent cells was significantly greater than that of HK JCM1149. Anti-LTA antibody and anti-scavenger receptor-A (SR-A) antibody selectively inhibited phagocytosis of HK L-137, as well as IL-12p40 production, by splenic adherent cells. Thus, a higher efficiency of phagocytosis of HK L-137 via SR-A for LTA is responsible for the potent IL-12p40 induction.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 25, Issue 2, April 2015, Pages 321–331
نویسندگان
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