کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2540791 | 1559761 | 2014 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Arg25Pro (c.915G>C) polymorphism of transforming growth factor β1 gene increases the risk of developing Graves' disease Arg25Pro (c.915G>C) polymorphism of transforming growth factor β1 gene increases the risk of developing Graves' disease](/preview/png/2540791.png)
• This study examined the possible association of TGFβ1 gene and GD.
• Leu10Pro (c.869T>C) and Arg25Pro (c.915G>C) polymorphisms were assayed by RFLP-PCR.
• No significant associations between GD and variant allele of Leu10Pro were observed.
• There was a significant increase of Arg25Pro C allele frequency in patients with GD.
• Arg25Pro CG + CC genotypes had 5.31-fold increased risk for GD according to GG.
BackgroundGraves' disease (GD) arises due to complex interactions between genetic and environmental factors. Transforming growth factor β1 (TGFβ1) is required to maintain immune homeostasis, and is implicated in lymphocyte infiltration, thyroid follicular cell hyperplasia, and production of autoantibody in the thyroid gland of patients with GD.AimThe aim of the present study was to investigate the possible association of Leu10Pro (c.869T>C) and Arg25Pro (c.915G>C) single nucleotide polymorphisms (SNPs) of TGFβ1 gene with the occurrence of GD.MethodsWe analyzed the genotype and allele frequencies of these SNPs in 171 patients with GD and 197 healthy controls using PCR-restriction fragment length polymorphism (RFLP).ResultsThe distribution of Leu10Pro (c.869T>C) genotype and allele frequencies in the control and GD groups were not significantly different. However, there was a significant increase of Arg25Pro (c.915G>C) C allele frequency in patients with GD compared with healthy controls (p < 0.0001, OR = 4.77, 95% CI = 3.32–7.03). In addition, C allele carrying subjects (CG + CC) had 5.31-fold increased risk for developing GD according to GG homozygotes (p < 0.0001, 95% CI = 3.43–8.44). No association between polymorphisms and GD phenotypes was observed.ConclusionThis study indicates that the Arg25Pro (c.915G>C) polymorphism of TGFβ1 gene may be related to occurrence of GD.
Journal: International Immunopharmacology - Volume 20, Issue 2, June 2014, Pages 366–369