کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2540906 1122622 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The therapeutic effects of daphnetin in collagen-induced arthritis involve its regulation of Th17 cells
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
The therapeutic effects of daphnetin in collagen-induced arthritis involve its regulation of Th17 cells
چکیده انگلیسی

ObjectivesDaphne odora var. marginata (D. marginata), an aiophyllus arbuscular plant, is one of the traditional Chinese medicines used to treat rheumatoid arthritis. This study investigated the therapeutic effects and mechanisms of daphnetin, an active monomer ingredient derived from D. marginata, on collagen-induced arthritis (CIA) in rats.MethodsThe effects of daphnetin on joint diseases were assessed by hematoxylin and eosin staining and radiographic and transmission electron microscopy. The protein and mRNA expression levels of T helper (Th)1/Th2/Th17-type cytokines in the spleen were determined by flow cytometry and quantitative real-time PCR.ResultsOur results showed that daphnetin significantly reduced paw swelling and was nontoxic in vivo at the tested doses. Synovial hyperplasia, joint destruction and chondrocyte degeneration in CIA rats were suppressed by daphnetin. Daphnetin treatment also reduced the levels of Th1/Th2/Th17 type cytokines in spleen lymphocytes in CIA rats. Moreover, the expression of Foxp3, which can down-regulate the activity of Th17 cells, was significantly increased in the daphnetin-treated groups.ConclusionsThese results suggest that daphnetin may have therapeutic effects in down-regulating Th17-type responses in CIA rats. The beneficial effects of daphnetin on CIA may be related to its inhibition of Th17 cell priming and activation.


► We designed to explore the therapeutic effects of daphnetin on collagen-induced arthritis in rats and its mechanisms.
► Daphnetin has function of anti-inflammation and regulation of Th17-type response in the CIA model.
► Daphnetin can promote protective immune responses in CIA rats.
► Daphnetin has a potential to be developed as a new type of treatment of RA.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 13, Issue 4, August 2012, Pages 417–423
نویسندگان
, , , , , , , ,