کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2541104 | 1122641 | 2013 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Icaritin exhibits anti-inflammatory effects in the mouse peritoneal macrophages and peritonitis model Icaritin exhibits anti-inflammatory effects in the mouse peritoneal macrophages and peritonitis model](/preview/png/2541104.png)
• The consequences of inhibiting Ca2 + by SKF in LPS/IFN-γ-activated macrophages.
• SKF inhibited the elevation of Ca2 + induced by ion, TG and LPS/IFN-γ, respectively.
• SKF inhibited the phagocytic ability and reduced NO and ROS production.
• SKF decreased the release of IL-6, MCP-1, TNF, IFN-γ, IL-10, but augmented IL-1β.
• SKF inhibited the translocation of NF-κB and induced ΔΨm loss.
Icaritin, an intestinal metabolite of prenylflavonoids from Herba Epimedii, has been known to regulate many cellular processes. The purpose of this study was to investigate the protective effects of icaritin on inflammation in lipopolysaccharide (LPS) stimulated mouse peritoneal macrophages in vitro and zymosan induced peritonitis model in vivo. The release of Nitric oxide (NO) was measured by a Griess reagent system. The phagocytosis, the expression of CD69, the production of inflammatory cytokines and the leukocytes numbers were determined by flow cytometry. The Ca2 + influx was recorded by confocal microscopy. The phosphorylation of p38, c-Jun N-terminal kinase (JNK), and extracellular signal-regulated kinase (ERK) was determined by Western blot. The results showed that icaritin significantly inhibited the NO, IL-6, IL-10 TNF-α, and MCP-1 production both in vitro and in vivo. Icaritin efficiently diminished the uptake of nonopsonized pHrodo™-labeled Escherichia coli bacteria on the LPS-stimulated macrophages. In addition, icaritin significantly inhibited the expression of CD69 on CD11b+ macrophages. Icaritin pretreatment significantly inhibited the elevation of intracellular Ca2 + induced by LPS. Furthermore, icaritin markedly decreased phospho-p38 and JNK protein expression in LPS-stimulated mouse peritoneal macrophages. In vivo study, it was also observed that icaritin prolonged survival of peritonitis mice, and inhibited massive leukocyte influx into the peritoneal cavity. These results suggest that icaritin possesses significant anti-inflammatory effects that may be mediated through the regulation of inflammatory cytokines and phosphorylation of p38 and JNK.
Journal: International Immunopharmacology - Volume 16, Issue 1, May 2013, Pages 41–49