Methylene chloride protects against cecal ligation and puncture-induced acute lung injury by modulating inflammatory mediators

Recent studies suggest that exogenously administered CO is beneficial for the resolution of acute pulmonary inflammation. In this study, we assessed the role of CO donor, methylene chloride (MC), on modulation of lung inflammation during sepsis. Acute lung injury in Sprague–Dawley rats was induced by cecal ligation and perforation (CLP). MC (100 mg/kg) was intragastrically administered 2 h before CLP induction. Lung tissues and lavage samples were isolated for biochemical determinations and histological measurements 10 h after CLP operation. In addition, we investigated survival rate with the other 40 rats. Intragastric administration with MC significantly decreased morbidity and mortality of CLP-induced ALI as confirmed by blinded histological changes, myeloperoxidase activity, mortality, and the content of TNF-α and IL-10. This protective effect could be abolished by an MC inhibitor, disulfiram. These results suggested that MC has obvious protective effects against CLP-induced ALI in rats. The mechanism of the protective effects partly involves modulating inflammatory mediators.