کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2541519 1122661 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inhibitory effects of parthenolide on antigen-induced microtubule formation and degranulation in mast cells
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Inhibitory effects of parthenolide on antigen-induced microtubule formation and degranulation in mast cells
چکیده انگلیسی

Pharmacological modulation of IgE-mediated mast cell activation is important to the development of anti-allergic reagents. In this study, we investigated the effects of parthenolide (PTL) on high-affinity IgE receptor (FcεRI)-induced degranulation in mast cells. PTL dose-dependently inhibited degranulation induced by IgE·antigen stimulation in RBL-2H3 cells and BMMCs. Although PTL is a potent NF-κB inhibitor by targeting IκB kinase complex, NF-κB inhibition by other IκB kinase inhibitors did not inhibit degranulation in mast cells. IgE·antigen-induced microtubule formation is well known to be critical for degranulation in mast cells. Immunocytochemical study with anti-α-tubulin antibody revealed that PTL significantly inhibited IgE·antigen-induced microtubule formation. However, PTL, as well as nocodazol, had no significant effects on degranulation in the fyn-deficient BMMCs, suggesting that inhibitory effects of PTL in the microtubule formation are fyn dependent. We further demonstrated that in vivo administration of PTL in mice strongly inhibited passive cutaneous anaphylaxis reaction. The present study provides a possibility to develop potent reagents against mast cell activation based on an inhibition of microtubule formation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 8, Issue 6, June 2008, Pages 874–880
نویسندگان
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