کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2541881 | 1559764 | 2009 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Valnemulin downregulates nitric oxide, prostaglandin E2, and cytokine production via inhibition of NF-κB and MAPK activity Valnemulin downregulates nitric oxide, prostaglandin E2, and cytokine production via inhibition of NF-κB and MAPK activity](/preview/png/2541881.png)
Valnemulin is a pleuromutilin antibiotic used in clinics for the treatment of various infections. We studied the in vitro anti-inflammatory effects of valnemulin and associated signal transduction mechanisms in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. We found that valnemulin inhibited nitric oxide (NO), prostaglandin E2 (PGE2), tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and increased interleukin-10 (IL-10) production. Inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression were also inhibited by valnemulin. We further observed that valnemulin prevented the LPS-induced NF-κB translocation from the cytoplasm into the nucleus. Valnemulin also blocked phosphorylation of three mitogen-activated protein kinases (MAPKs): extracellular signal receptor-activated kinase (ERK) 1/2, p38, and c-Jun N-terminal kinase (JNK). Our data indicate that valnemulin may have therapeutic anti-inflammatory effects independent of its antibacterial activity.
Journal: International Immunopharmacology - Volume 9, Issues 7–8, July 2009, Pages 810–816