In hemocytes from Mytilus galloprovincialis Lmk., treatment with corticotropin or growth factors conditions catecholamine release

The cells in charge of the innate immune response in the sea mussel Mytilus galloprovincialis Lmk. are the hemocytes, which have the capacity to release catecholamines when subjected to stressing conditions. Hemocytes were kept in culture before stimulation. That is, their behaviour was not studied immediately after extraction from the mollusc, as happens in most studies. This avoids the interference and variability caused by the conditions in which mussels may be when collected.This work describes the great variability found in the pattern of catecholamine release when the hemocytes are stimulated with either corticotropins or growth factors. Dopamine, adrenaline and noradrenaline release differs with each of the inducers assayed, with stimulation time and with the season of hemocyte collection. One of the results presented is particularly remarkable; such is the great amount of adrenaline and noradrenaline released to the medium when the hemocytes obtained in summer are stimulated with transforming growth factor-β1 (TGF-β1) for 60 min.