کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2542520 1122708 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Isolation and characterization of an anti-complementary polysaccharide D3-S1 from the roots of Bupleurum smithii
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Isolation and characterization of an anti-complementary polysaccharide D3-S1 from the roots of Bupleurum smithii
چکیده انگلیسی
The preliminary data from hemolytic assays indicated that the hot-water extract of the roots of Bupleurum smithii had anti-complementary activity. Further bioactivity-guided fractionation led to the isolation of D3-S1, a homogeneous form of acidic polysaccharide. D3-S1 was a branched polysaccharide with average molecular weight about 2,000,000 Da, composed of Ara, Gal and GalA in the ratio of 2.6:1.0:1.2, along with trace of Rha, Glc, Xyl and Man. Methylation analysis and NMR identified the linkages of the residues of D3-S1. Functional analysis showed that D3-S1 inhibited complement activation on both the classic and alternative pathways with CH50 value of 0.34 ± 0.02 mg/ml and AP50 value of 0.081 ± 0.003 mg/ml, respectively. Preliminary mechanism studies by using complement component depleted-sera indicated that D3-S1 selectively interacts with C1s, C3 and C4, but not C1q, C1r, C2, C5 and C9. The results suggested that D3-S1 could be of potential benefits in treatment of the complement-associated diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 7, Issue 2, February 2007, Pages 175-182
نویسندگان
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