کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2544500 1560372 2016 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cancer cell specific cytotoxic effect of Rhoeo discolor extracts and solvent fractions
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی داروشناسی
پیش نمایش صفحه اول مقاله
Cancer cell specific cytotoxic effect of Rhoeo discolor extracts and solvent fractions
چکیده انگلیسی

Ethnopharmacological relevanceTraditional or folk medicine has led to the discovery of important bioactive substances used in several health-related areas. Phytochemicals in Rhoeo discolor (R. discolor) extracts have proven to have important cancer cell specific cytotoxic activity. In the present research, we determined the cytotoxic effect of extracts of R. discolor, a plant commonly used in Mexico for both medicinal and ornamental purposes.Aim of the studyWe evaluated the cytotoxic effects against three representative human cancer cell lines: HT-29 colon cancer, Hep-G2 liver cancer and PC-3 prostate cancer cell lines, as well as a control fibroblast cell line NIH 3T3.Materials and methodsTen different crude extracts were tested along with fractions derived from the five most bioactive crude extracts. Analytical data, HPLC-MS-TOF, revealed a high content of phenolic compounds such as anthocyanins, ferulic, vanillic, chlorogenic and p-coumaric acid in the extracts. Phenolic compounds have previously been reported as health beneficial with antioxidant and potential cancer specific cytotoxic effects.ResultsStudies revealed that low concentrations of these crude bioactive extracts (10 µg/ml) and their fractions (50 µg/ml) were effective as cancer specific cytotoxic agents, since they caused a significant proliferation inhibition on cancer cell lines (up to 94.2% in HT-29, 92.9% in Hep-G2 and 61.8% in PC-3 of apoptosis induction) with little harm to the control cell line (no higher than 28.3% apoptosis induction), and, importantly, the most effective extracts were mainly water, methanol and ethanol based.ConclusionsThese results suggest that a diet containing these compounds may function as a medical aid or chemoprotective.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Ethnopharmacology - Volume 190, 22 August 2016, Pages 46–58
نویسندگان
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