کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2545034 | 1560399 | 2015 | 9 صفحه PDF | دانلود رایگان |
Ethnopharmacological relevanceThe aqueous extract of the plant Malmpyrum pratense (Mp), is widely used in traditional medicine as a sedative, yet the biological basis of its action is not known.Aim of the studyThe effects of Mp on network activity and intrinsic and synaptic properties were studied in cultured hippocampal neurons in an attempt to analyze its mode of action.Materials and methodsDissociated cultures of rat hippocampal neurons were used. Spontaneous network activity was assessed by variations in intracellular [Ca2+] concentrations, reflecting action potential discharges. Individual neuronal synaptic activity was measured by patch clamp recordings from similar neurons. The effect of exposure to different concentrations of Mp and some of its main ingredients was measured.ResultsMp produced complex, dose dependent, reversible effects on network activity, increasing it with low concentrations, and decreasing it at high concentrations. Individual flavonoids contained in Mp mimicked the effects of the extract, both for the facilitating and suppressing effects of the extract. Electrophysiologically, Mp caused a reduction in spontaneous activity, but did not affect membrane properties of individual patch clamped neurons, nor did it affect mEPSCs recorded from these neurons. However, a transient increase in reactivity to pulse application of GABA was evident.ConclusionsThese results suggest that a main sedative effect of Mp is on GABAergic neurotransmission in cultured hippocampal neurons.
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Journal: Journal of Ethnopharmacology - Volume 163, 2 April 2015, Pages 220–228