کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2545988 | 1124012 | 2010 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Constituents isolated from Cordyceps militaris suppress enhanced inflammatory mediator's production and human cancer cell proliferation Constituents isolated from Cordyceps militaris suppress enhanced inflammatory mediator's production and human cancer cell proliferation](/preview/png/2545988.png)
Aim of the studyThe purpose of this study is to isolate the pure compounds from the extracts of Cordyceps militaris obtained through solid-state cultivation process, and evaluate their anti-inflammatory and anticancer properties.Materials and methodsSilica gel column chromatographic purification of Cordyceps militaris extracts resulted in the isolation of 10 pure compounds (1–10). The compounds 1–10 were examined for their growth inhibitory properties against nitric oxide (NO), tumor necrosis factor (TNF)-α and interleukin (IL)-12 enhanced production from LPS/IFN-γ-stimulated macrophages. Additionally, the anti-proliferation effects of 1–10 on human cancer cell lines, colon (colon 205), prostate (PC-3), and hepatoma (HepG2) cells were also analyzed.ResultsCompound 8 displayed potent growth inhibition on NO, TNF-α and IL-12 production with an IC50 value of 7.5, 6.3, and 7.6 μg/ml, respectively. A similar inhibitory trend on these inflammatory mediators was observed for 3, 7, 9 and 10 with an IC50 values ranging from 10.8 to 17.2 μg/ml. On the other hand, compounds 3 and 8 were potent anti-proliferative agents with an IC50 value of 35.6 and 32.6 μg/ml toward PC-3 and colon 205 cell lines, respectively. The compounds 1 and 2 showed potent anti-proliferation in PC-3 and colon 205 cells, while only 3 displayed such effect in HepG2 cells.ConclusionThe present study provides scientific supporting information for the ethnopharmacological use of Cordyceps militaris as an anti-inflammatory and anticancer agent.
In traditional Chinese medicine, Cordyceps militaris is widely used for various ethnomedical conditions including inflammation and cancer complications. In this report, silica gel column chromatographic purification of Cordyceps militaris extracts resulted in the isolation of 10 pure compounds. These compounds displayed potent growth inhibition on nitric oxide, tumor necrosis factor-α and interleukin-12 production from LPS/IFN-γ-stimulated macrophages, in addition to their anti-proliferation effect against human cancer cells.Figure optionsDownload as PowerPoint slide
Journal: Journal of Ethnopharmacology - Volume 131, Issue 2, 15 September 2010, Pages 363–367