Anti-inflammatory principles of Suregada multiflora against nitric oxide and prostaglandin E2 releases

Aim of the studyThe stem bark of Suregada multiflora and the isolated compounds were carried out to investigate for anti-inflammatory activity.Materials and methodsThe stem bark of Suregada multiflora and its isolated compounds were tested for their anti-inflammatory effects against lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostraglandin E2 (PGE2) releases in RAW264.7 cells as well as the anti-inflammatory mechanism on mRNA expression of the active compound (5, helioscopinolide A).ResultsThe extract of Suregada multiflora possessed potent NO inhibitory effect with an IC50 value of 8.6 μg/ml. Among the isolated compounds, helioscopinolide A (5) exhibited the highest activity against NO release with an IC50 value of 9.1 μM, followed by helioscopinolide C (6) and suremulol D (2) with IC50 values of 24.5 and 29.3 μM, respectively. The IC50 value of 5 against PGE2 production was found to be 46.3 μM. The mechanism in transcriptional level of compound 5 was found to inhibit iNOS and COX-2 mRNA expressions in dose-dependent manners.ConclusionsThe present study may support the traditional use of Suregada multiflora stem bark for treatment of inflammatory-related diseases.

Helioscopinolide A (5) isolated from the bark of Suregada multiflora exhibited potent activity against NO and PGE2 releases with IC50 values of 9.1 and 46.3 μM, respectively. Compound 5 inhibited iNOS and COX-2 mRNA expression in a dose-dependent manner.Figure optionsDownload as PowerPoint slide