کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2546132 | 1124017 | 2009 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Effect of Pinus massoniana bark extract on IFN-γ-induced ICAM-1 expression in HaCaT human keratinocytes Effect of Pinus massoniana bark extract on IFN-γ-induced ICAM-1 expression in HaCaT human keratinocytes](/preview/png/2546132.png)
Aims of the studyPinus massoniana bark extract (PMBE) with known anti-oxidant activity is comprised of various flavonoids including several bioactive compounds. We found that PMBE contains 1.27% taxifolin, a well-studied compound with known anti-inflammatory activity. Therefore, in the present study, we evaluated the effects of PMBE and taxifolin on intercellular adhesion molecule-1 (ICAM-1) expression.Materials and MethodsPMBE and taxifolin were prepared. After HaCaT cells were pre-treatmented with PMBE and taxifolin, HaCaT cells were treatmented with 1000 U/ml IFN-γ for 24 h.ResultsTreatment of HaCaT cells with 1000 U/ml IFN-γ for 24 h markedly increased ICAM-1 expression. However, PMBE pre-treatment (40 μg/ml for 24 h) significantly inhibited IFN-γ-induced ICAM-1 expression. In equal concentrations of taxifolin, PMBE-mediated inhibition of ICAM-1 mRNA and protein expression was greater than taxifolin mediated-inhibition, and the front on inhibition of ICAM-1 protein expression was 2.24–2.30-fold of the latter. When cells were treated with both compounds at a concentration of 40 μg/ml, PMBE-mediated inhibition of ICAM-1 mRNA was also greater than taxifolin-mediated inhibition and PMBE on inhibition of ICAM-1 protein expression was 2.60–3.00-fold the inhibition mediated by taxifolin.ConclusionsPMBE including additional bioactive compounds may possibly synergize to inhibit transcription and translation of inducible ICAM-1expression and PMBE was greater than monomeric flavonoid taxifolin. These results indicate that PMBE exhibits great potential as a therapeutic treatment for inflammatory skin diseases.
Journal: Journal of Ethnopharmacology - Volume 122, Issue 1, 25 February 2009, Pages 48–53