Molecular pharmacological basis of the YiSui ShenXu Granule in β-thalassemia therapy

ObjectivesTo study the molecular pharmacological basis of the YiSui ShenXu Granule, a complex prescription of the Chinese traditional medicine used to treat β-thalassemia.MethodsReal-time quantitative PCR method had been applied to analyze the genes expression: γ-globin, Ckit, EpoR, Spi, FKLF, GATA1 and GATA2 in K562 cell treated and untreated with this complex prescription and its each single herbal medicine.ResultsThe results showed that this complex prescription increased the γ-globin, EpoR, Spi and FKLF expression and decreased the Ckit, GATA1 and GATA2 expression. And all single herbal medicines of this complex prescription could change some of those gene expressions, but not the same as the complex prescription. Even that, this study results indicated that the YiSui ShenXu Granule has its molecular pharmacological basis in treating β-thalassemia.