کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2546614 1124031 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Armillariella mellea induces maturation of human dendritic cells without induction of cytokine expression
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی داروشناسی
پیش نمایش صفحه اول مقاله
Armillariella mellea induces maturation of human dendritic cells without induction of cytokine expression
چکیده انگلیسی

Ethnopharmacological relevanceArmillariella mellea, an edible and medicinal mushroom possessing immuno-modulating potential, has been frequently used for the treatment of infectious diseases or cancers.Aim of the studyIn order to elucidate immune-regulatory mechanisms of Armillariella mellea, we investigated the effect of water-soluble components from Armillariella mellea (AME) on the regulation of human dendritic cell (DC) maturation and activation.Materials and MethodsImmature DCs (iDCs) were prepared by differentiating human peripheral blood CD14-positive cells with GM-CSF and IL-4. Then, iDCs were treated with AME at 2–20 μg/ml for 48 h and subjected to flow cytometry to analyze the expression of DC markers. Dextran-FITC uptake assay and enzyme-linked immunosorbent assay were performed to examine the endocytic capacity of AME-stimulated DC and their production of cytokines, respectively.ResultsiDCs stimulated with AME showed representative features during DC maturation such as up-regulated expression of CD80, CD83, CD86, both MHC class I and II molecules, and CD205, with a simultaneous decrease in the expression of CD206 and the endocytic capacity. Interestingly, AME was not able to induce the production of TNF-α, IL-12p40, or IL-10, whereas lipopolysaccharides induced a substantial increase of all of the cytokines.ConclusionArmillariella mellea induces maturation of human DCs through a unique mechanism without inducing cytokine expression.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Ethnopharmacology - Volume 119, Issue 1, 2 September 2008, Pages 153–159
نویسندگان
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