کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2547871 1124072 2008 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Anticomplementary principles of a Chinese multiherb remedy for the treatment and prevention of SARS
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی داروشناسی
پیش نمایش صفحه اول مقاله
Anticomplementary principles of a Chinese multiherb remedy for the treatment and prevention of SARS
چکیده انگلیسی

Aim of the studyTo elucidate the anticomplementary principles of a Chinese multiherb remedy used for the treatment and prevention of SARS, which contains five ingredients of Herba Houttuyniae, Flos Chrysanthemi Indici, Herba Artemisiae Scopariae, Herba Eupatorii and Fructus Tsaoko.Materials and methodsThe anticomplementary activity was evaluated from hemolytic assays through the classical pathway (CP) and the alternative pathway (AP) of complement system in vitro. Compounds were isolated using bioactivity-guided fractionation and tested in vitro for their complement-inhibiting properties on the CP and AP. HPLC-DAD-ESI-MS was used to assign the isolated compounds in the five ingredient herbs.Results15 compounds, including chlorogenic acid (1), rutin (2), hyperoside (3), p-hydroxyacephenone (4), scopoletin (5), quercitrin (6) (3R,4R,6S)-3,6-dihydroxy-1-menthene (7), acaciin (8), scoparone (9), luteolin (10), quercetin (11), apigenin (12), acacetin (13), aristolactam (14), and apigenin-7,4′-dimethyl ether (15) were isolated and identified. Nine flavonoids (2, 3, 6, 8, 10–13, 15) were found to show inhibitory effects towards the CP and AP of complement system, luteolin (10) was the most potent with the CH50 and AP50 values of 0.19 and 0.17 mM. The bioactive flavonoids were mainly derived from Herba Houttuyniae, Flos Chrysanthemi Indici and Herba Artemisiae Scopariae.ConclusionsA Chinese multiherb remedy used for the treatment and prevention of SARS has robust inhibitory effect on complement system through the CP and AP, and its anticomplementary activity could be attributed to the flavonoids present in some ingredient herbs. Luteolin could be a potential anticomplementary agent.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Ethnopharmacology - Volume 117, Issue 2, 8 May 2008, Pages 351–361
نویسندگان
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