A comparison of three rapid and accurate bioluminescent antibiotic susceptibility tests

IntroductionRapid determination of microorganisms' antibiotic susceptibility remains a great challenge for the clinical diagnosis of infectious diseases. In this study, we compare three rapid bioluminescent antibiotic susceptibility tests with the conventional normalised microdilution method.MethodsThe susceptibility of two bacterial and two fungal strains to a 6 h incubation with one of eight standard antibiotics was investigated by the ATP, AK and QNO bioluminescent methods.ResultsThree rapid and accurate antibiotic susceptibility testing were investigated performing ATP, AK and QNO bioluminescent assays. All the results were in accordance with those obtained using a standard microdilution assay. All these methods were sensitive and faster than the previously well-known ones.DiscussionThe development of bioluminescent determination methods could lead to accurate and useful antibiotic susceptibility testing.