کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2549670 1560494 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Closed-chest experimental porcine model of acute myocardial infarction–reperfusion
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی داروشناسی
پیش نمایش صفحه اول مقاله
Closed-chest experimental porcine model of acute myocardial infarction–reperfusion
چکیده انگلیسی

IntroductionProgress in cardiovascular regenerative medicine research requires the availability of appropriate experimental animal models that are as close to humans as feasible. Our objective was to assess the validity of a porcine endovascular model of myocardial infarction and reperfusion.MethodsFifteen domestic pigs (Large White race) were anesthetized and pre-medicated with amiodarone. Endovascular fluoroscopy-guided coronary procedures were performed to occlude the mid-left anterior descending artery using a coronary angioplasty balloon. Occlusion was confirmed by angiography and electrocardiography. After 75 min the balloon catheter system was withdrawn and the presence of reperfusion flow was verified. The animals were sacrificed after 1 and 2 weeks of follow-up, the hearts were explanted, and the extent of myocardial infarction with respect to the left ventricle was quantified.ResultsOverall survival rate was 67%. Five animals died prematurely: 3 showing signs of heart failure, 1 had reperfusion failure (final TIMI flow grade 1) and 1 succumbed to acute stress. The most common adverse event was ventricular fibrillation (87% of the animals) and defibrillation was effective in all affected animals. The extent of myocardial infarct in the animals followed-up for 1 and 2 weeks was similar (20.4 ± 4.3% vs. 20.9 ± 2.8%, respectively; p = 0.8) but was significantly greater in the animals that died prematurely (29.5 ± 3.6%, p = 0.02).ConclusionsThe endovascular porcine model we have explored constitutes a feasible and reproducible alternative for the evaluation of human myocardial infarction and reperfusion.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmacological and Toxicological Methods - Volume 60, Issue 3, November–December 2009, Pages 301–306
نویسندگان
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