کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2568820 1128486 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bisphenol A differentially activates protein kinase C isoforms in murine placental tissue
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Bisphenol A differentially activates protein kinase C isoforms in murine placental tissue
چکیده انگلیسی


• The pollutant bisphenol A differentially activated PKC isoforms in the placenta.
• CRE-binding activity in the nuclear protein of placenta was increased.
• Bisphenol A induces CRH mRNA expression in mice.

Bisphenol A is utilized to make polycarbonate plastics and is an environmental pollutant. Recent research has indicated that it is an endocrine disruptor and may interfere with reproductive processes. Our lab has previously shown that bisphenol A could regulate corticotrophin releasing hormone and aromatase in cultured placental cells. In the present study, the effect of bisphenol A on these two genes in the placenta was investigated in mice. Pregnant ICR mice were gavaged with bisphenol A at 2, 20 and 200 mg/kg body weight/day from E13 to E16 and were euthanized at E17. Compared to the control mice, increased plasma estrogen and corticotrophin releasing hormone were observed in bisphenol A-treated mice. Messenger RNA quantification indicated that placental crh but not cyp19 was induced in mice treated with bisphenol A. Tracking the related signaling pathway, we found that protein kinase C ζ/λ and δ were activated in the placentas of bisphenol A-treated mice. As the gene promoter of crh contains CRE and the half site of ERE, either phospho-PKC or estrogen could stimulate the gene transactivation. These results indicate that bisphenol A might increase plasma concentrations of estradiol, testosterone, corticotrophin releasing hormone and placental phospho-PKC ζ/λ and δ in mice. Ultimately, the incidence of premature birth in these mice could increase.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology and Applied Pharmacology - Volume 269, Issue 2, 1 June 2013, Pages 163–168
نویسندگان
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