کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2569323 | 1128523 | 2013 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Potent inhibition of human neutrophil activations by bractelactone, a novel chalcone from Fissistigma bracteolatum Potent inhibition of human neutrophil activations by bractelactone, a novel chalcone from Fissistigma bracteolatum](/preview/png/2569323.png)
Fissistigma bracteolatum is widely used in traditional medicine to treat inflammatory diseases. However, its active components and mechanisms of action remain unclear. In this study, (3Z)-6,7-dihydroxy-4-methoxy-3-(phenylmethylidene)-5-(3-phenylpropanoyl)-1-benzofuran-2(3H) (bractelactone), a novel chalcone from F. bracteolatum, showed potent inhibitory effects against superoxide anion (O2−) production, elastase release, and CD11b expression in formyl-l-methionyl-l-leucyl-l-phenylalanine (FMLP)-induced human neutrophils. However, bractelactone showed only weak inhibition of phorbol myristate acetate-caused O2− production. The peak cytosolic calcium concentration ([Ca2 +]i) was unaltered by bractelactone in FMLP-induced neutrophils, but the decay time of [Ca2 +]i was significantly shortened. In a calcium-free solution, changes in [Ca2 +]i caused by the addition of extracellular Ca2 + were inhibited by bractelactone in FMLP-activated cells. In addition, bractelactone did not alter the phosphorylation of p38 MAPK, ERK, JNK, or AKT or the concentration of cAMP. These results suggest that bractelactone selectively inhibits store-operated calcium entry (SOCE). In agreement with this concept, bractelactone suppressed sustained [Ca2 +]i changes in thapsigargin-activated neutrophils. Furthermore, bractelactone did not alter FMLP-induced formation of inositol 1,4,5-triphosphate. Taken together, our results demonstrate that the anti-inflammatory effects of bractelactone, an active ingredient of F. bracteolatum, in human neutrophils are through the selective inhibition of SOCE.
Figure optionsDownload high-quality image (216 K)Download as PowerPoint slideHighlights
► Bractelactone isolated from Fissistigma bracteolatum.
► Bractelactone inhibited FMLP-induced human neutrophil activations.
► Bractelactone had no effect on IP3 formation.
► Bractelactone did not alter MAPKs, AKT, and cAMP pathways.
► Bractelactone inhibited store-operated calcium entry.
Journal: Toxicology and Applied Pharmacology - Volume 266, Issue 3, 1 February 2013, Pages 399–407