کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2571290 | 1128626 | 2007 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nicotine promotes cell proliferation via α7-nicotinic acetylcholine receptor and catecholamine-synthesizing enzymes-mediated pathway in human colon adenocarcinoma HT-29 cells
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کلمات کلیدی
AADCRT-PCRDβHdopamine-β-hydroxylaseα7-nicotinic acetylcholine receptorβ-adrenoceptorsPNMTaromatic-l-amino acid decarboxylaseadrenaline - آدرنالینtyrosine hydroxylase - تیروزین هیدروکسیلازColon cancer - سرطان کولونPhenylethanolamine N-methyltransferase - فنیلتانولامین N-متیل ترانسفرازNicotine - نیکوتین reverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوس
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Cigarette smoking has been implicated in colon cancer. Nicotine is a major alkaloid in cigarette smoke. In the present study, we showed that nicotine stimulated HT-29 cell proliferation and adrenaline production in a dose-dependent manner. The stimulatory action of nicotine was reversed by atenolol and ICI 118,551, a β1- and β2-selective antagonist, respectively, suggesting the role of β-adrenoceptors in mediating the action. Nicotine also significantly upregulated the expression of the catecholamine-synthesizing enzymes [tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DβH) and phenylethanolamine N-methyltransferase]. Inhibitor of TH, a rate-limiting enzyme in the catecholamine-biosynthesis pathway, reduced the actions of nicotine on cell proliferation and adrenaline production. Expression of α7-nicotinic acetylcholine receptor (α7-nAChR) was demonstrated in HT-29 cells. Methyllycaconitine, an α7-nAChR antagonist, reversed the stimulatory actions of nicotine on cell proliferation, TH and DβH expression as well as adrenaline production. Taken together, through the action on α7-nAChR nicotine stimulates HT-29 cell proliferation via the upregulation of the catecholamine-synthesis pathway and ultimately adrenaline production and β-adrenergic activation. These data reveal the contributory role α7-nAChR and β-adrenoceptors in the tumorigenesis of colon cancer cells and partly elucidate the carcinogenic action of cigarette smoke on colon cancer.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology and Applied Pharmacology - Volume 221, Issue 3, 15 June 2007, Pages 261-267
Journal: Toxicology and Applied Pharmacology - Volume 221, Issue 3, 15 June 2007, Pages 261-267
نویسندگان
Helen Pui Shan Wong, Le Yu, Emily Kai Yee Lam, Emily Kin Ki Tai, William Ka Kei Wu, Chi Hin Cho,